Services
Magnify Biosciences provides comprehensive services from specimen prep to nanoimaging analysis, aiding drug development and disease research with our Magnify-validated antibodies and expertise, delivering nanoscale insights swiftly and precisely.
II. Initial Consultation
- Discuss project needs, sample types, resolution requirements, and labeling options.
- Confirm compatibility of antibodies with Magnify’s protocols.
- Magnify provides an overview of available services
IV. Sample Preparation and Shipping
- Prepare samples according to Magnify’s guidelines.
- Ship samples to Magnify with proper fixation and handling.
VI. Result Delivery
- Review results with Magnify’s technical team.
- Request additional services if needed (e.g., further labeling, ROI comparisons).
- Receive final imaging data and analysis reports.
I. Contact Magnify
Email or complete the Magnify service consultation form
III. Submit Service Request
- Finalize service request with selected options.
- Receive cost estimate and timeline for service completion.
V. Sample Processing and Imaging
- Magnify processes samples based on selected options.
- Imaging and analysis services are performed, including volumetric imaging and specialty imaging, such as SOFI, if requested.
- The Magnify team can optionally ship the expanded sample back in the selected format.
Magnify enables precise mapping of sub-organelle localization of biologics drug candidates
This Magnify image was captured using a Nikon Eclipse Ti2 with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.15 water immersion objective. The sample, Magnify-processed Human Bronchial Epithelial (HBE) cells, was stained with DAPI (blue), CFTR (green), and SNAP23 (red) to assess changes in protein expression and localization in response to increasing doses of Cystic Fibrosis (CF) drug treatment.
Magnify enables precise mapping of sub-organelle localization for biologics drug candidates, offering valuable insights into their mechanisms of action. CFTR mislocalization, caused by mutations, is the primary cause of cystic fibrosis. Traditional optical microscopes often lack the resolution to confirm whether biologics drugs correctly target specific subcellular structures, such as the plasma membrane. However, using Magnify, we were able to visualize the localization of a new biologics candidate for treating cystic fibrosis and confirm the restoration of drug targets at the plasma membrane. Magnify revealed dose-dependent improvements in the membrane localization of key proteins—CFTR (green) and SNAP23 (red)—with nanoscale precision, using only a conventional confocal microscope. This approach advances our ability to study biologics at a sub-organelle level, enhancing drug development and discovery efforts.
Revolutionizes neural research with rapid 3D synapse mapping, bypassing traditional 2D layering technique by EM.
This Magnify image was acquired using a Nikon Eclipse Ti2 epifluorescence microscope equipped with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.2 water immersion objective. The sample, an expanded human tissue section, was stained with synaptophysin (yellow) and PSD95 (red).
Magnify can image 3D synaptic ultrastructure and protein markers with a fraction of the time and cost, accelerating the mapping of brain connectome. Left panel: 3D mapping using EM requires slicing tissue into tens of thousands of layers for 2D imaging, then compiling them into a 3D composite. Right panel: In contrast, Magnify’s rapid 3D mapping with detailed ultrastructure (the first column) and molecular specificity (the second column) revolutionizes our understanding of synaptic function and neural connectivity. The visualization of interactions, such as synaptophysin(yellow) with PSD95(red), is crucial for uncovering the mechanisms of learning and memory, and identifying disruptions linked to neurological
Achieve 15 nm resolution with Magnify-SOFI on conventional microscopes.
This Magnify-SOFI image was captured using a Nikon Eclipse Ti2 with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.15 water immersion objective. Resolution ~15 nm. The sample, a fully expanded section of the ependymal cell lining from the adult mouse brain, was stained with NHS-ATTO-488 and rendered in 3D to visualize the intricate structure of ependymal cilia and basal bodies.
High-resolution 3D imaging of ependymal cilia and basal bodies is essential for understanding their role in cerebrospinal fluid circulation and brain homeostasis. Magnify-SOFI allows detailed visualization of these structures without needing a expensive and slow serial block-face scanning electron microscope, making it a powerful and accessible tool for studying ciliary function.
Detect ultrastructural changes and reveal 3D cancer morphology before traditional methods.
This Magnify image was acquired using a Nikon Eclipse Ti2 epifluorescence microscope equipped with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.2 water immersion objective. The sample, an expanded human urinary bladder cancer tissue section, was stained with DAPI (white), CEPN-B (cyan), TelC (magenta), and WGA (yellow). Magnify achieved 3D imaging of the cancer tissue at an expansion factor of 3.8× in PBS, enabling detailed visualization of ultrastructural changes in cancer morphology.
Magnify’s capability to detect 3D cancer morphology at an ultrastructural level, often before traditional methods, is invaluable for early cancer diagnosis and understanding tumor progression. This enhanced resolution allows for the detailed observation of chromosomal structures and cellular architecture within tumors, providing critical insights that can inform more precise therapeutic approaches.
Fast, cost-effective 3D chromatin studies with super-resolution.
This Magnify image was acquired using a Nikon Eclipse Ti2 epifluorescence microscope equipped with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 63×/1.2 water immersion objective. It achieved 24 nm resolution with DAPI staining.
Magnify enables precise 3D imaging of dividing cell nuclei, revealing chromosomal structures down to the nucleosome level. This is achieved much faster than traditional 3D super-resolution techniques by leveraging the speed and 3D optical sectioning capabilities of spinning disk confocal microscopy. This efficiency is crucial for studying chromosome organization during cell division, offering valuable insights into genetic disorders and cancers associated with chromosomal anomalies.
View multiplexed molecular labels with nanoscale ultrastructural detail.
This image was captured using the Magnify expansion microscopy technique on a Nikon Eclipse Ti2 epifluorescence microscope, equipped with a CSU-W1 spinning disk confocal module and an Andor v.4.2 Zyla sCMOS camera, using a 63×/1.2 water immersion objective.
Magnify not only enhances resolution but also allows for multiplexed imaging with ten or more colors, far surpassing traditional fluorescence imaging capabilities. This makes it possible to simultaneously visualize and analyze multiple proteins and cellular structures within the same sample, providing comprehensive insights into complex biological processes, such as pathogen-host interactions and protein co-localization, at an unprecedented level of detail.
Ultrastructural imaging capabilities offer significant potential for many translational studies
This Magnify image was captured using a Nikon Eclipse Ti2 with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.15 water immersion objective. The sample, a fully expanded human FFPE kidney tissue, was stained with DAPI (magenta), ACTN4 (orange), and WGA (blue), and rendered in 3D to showcase the detailed architecture of renal structures.
Visualizing podocyte foot processes in 3D is crucial for understanding renal pathology and diagnosing kidney diseases. Magnify allows for high-resolution imaging of these delicate structures without the need for specialized microscopes, offering a powerful and accessible tool for renal pathology. By providing clear views of critical renal components like ACTN4 and WGA-labeled areas, Magnify enhances our ability to study kidney function and pathology, leading to better insights into disease mechanisms and potential treatments.