The Magnify Expansion Kit immobilizes various biomolecules to a hydrogel, enabling multi-scale isotropic expansion in PBS and water. This process enhances imaging resolution using your existing imaging methods. It is designed for ease of use, making it ideal for both first-time and experienced users across multiple sample types including fixed culture cells, freshly preserved tissue sections, FFPE slides and organoids etc.
Magnify kits are optimized for the expansion of culture cells, organoids and sectioned tissues, including challenging specimens like FFPE tissues, making them highly versatile across various biological samples.
Magnify ensures the preservation of a wide range of biomolecules with high efficiency, enabling post-expansion staining with excellent signal-to-noise.
Expanded tissues using Magnify are compatible with a wide range of microscopes, including confocal, light sheet, and super-resolution systems like STED and SIM. This broad compatibility enables detailed imaging of cellular and subcellular structures.
Magnify offers highly efficient tissue expansion within one day, making it suitable for both routine and complex studies.
Magnify kits offer a tunable expansion factor, expanding tissues by 3.5x in 1x PBS, and up to 11x in pure water. This adjustability allows researchers to fine-tune expansion based on their specific experimental goals, whether prioritizing resolution or tissue handling.
Magnify-expanded tissues maintain high mechanical strength and structural integrity, ensuring easy handling and the preservation of delicate cellular architecture, which are crucial for accurate downstream analyses.
Magnify kits are designed for consistency and uniform expansion results, ensuring reliable outcomes across different specimen types.
Magnify ensures that critical lipid and protein are preserved during the expansion process, enabling comprehensive molecular studies while maintaining the tissue ultrastructure context.
A fast, easy-to-use, and versatile kit that is compatible with any microscope and accessible to everyone.
These images compare mouse brain tissue captured with and without Magnify processing using Cephla’s SQUID system (https://cephla.com/). Both samples were stained with DAPI, synaptophysin (green), homer (red), and LEL (white), and imaged using a CrestOptics Cicero confocal spinning disk module, IMX571 camera, and a 63×/1.2 water immersion objective. The integration of Magnify enhances the imaging resolution and clarity, revealing finer details of neuronal and synaptic structures.
The application of Magnify significantly improves visualization of critical synaptic proteins such as synaptophysin and homer, providing a more detailed and comprehensive view of synaptic organization and neural connectivity. This enhanced imaging facilitates deeper insights into brain function and the underlying mechanisms of neurological disorders. By combining Magnify with accessible and high-throughput systems like Cephla’s SQUID, researchers can achieve superior image quality without reliance on specialized or bespoke microscopy setups, accelerating advances in neuroscience research.
This Magnify image was captured using a Nikon Eclipse Ti2 with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.15 water immersion objective. The sample was stained with Tom20 (magenta) and pan-protein stain (black and white), providing an electron microscopy-like context.
Using Magnify’s up to 11-fold expansion and achieving resolutions of up to 15 nm, this image precisely localizes Tom20 within the subcellular architecture of mitochondria. This capability highlights Magnify’s power to pinpoint the exact subcellular locations of proteins, including potential drug targets, and to illustrate how drugs interact within cells. Such detailed imaging is essential for advancing our understanding of cellular processes and the mechanisms of drug action.
This Magnify image was captured using a Nikon Eclipse Ti2 with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.15 water immersion objective. The sample, stained with DAPI (white), anti-GFP (blue), synaptophysin (magenta), and PSD95 (green), was rendered in 3D to reveal intricate neuronal architecture.
Studying detailed synaptic structures is key to mapping neuronal connections and advancing our understanding of connectomes. Magnify delivers high-resolution 3D imaging of SST neurons, revealing synaptic components like synaptophysin and PSD95. Its compatibility with genetically encoded tags, such as GFP, makes it an ideal tool for exploring brain connectivity and synaptic function, providing valuable insights into neurological health and disease.
This Magnify image was captured using a Nikon Eclipse Ti2 with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 10×/0.3 objective. The sample, a ~85 µm thick mouse colon section stained with pan-protein ATTO647N NHS-ester (magenta), was expanded to enhance both resolution and tissue clarity.
Unlike other tissue clearing methods that can take days or even weeks, Magnify achieves effective clearing and expansion within a single day. Traditional PFA-fixed tissues, especially those thicker than 30 microns, are often difficult to image due to high autofluorescence and light scattering. After Magnify expansion, the tissue becomes nearly transparent, allowing for much clearer and more detailed imaging, making it the superior choice for rapid and reliable results.
This µMagnify image was captured using a Nikon Eclipse Ti2 with a CSU-W1 spinning disk confocal module, Andor v.4.2 Zyla sCMOS camera, and a 40×/1.15 water immersion objective. The sample, a human cornea infected with Candida albicans, was post-expansion stained with DAPI (cyan), Tomato lectin LEL (yellow), and NHS-ester (magenta), and rendered in 3D to visualize detailed pathogen-host interactions within the dense collagen matrix of the cornea.
Studying these intricate pathogen interactions at the single-cell level is critical for understanding the mechanisms of keratitis. µMagnify provides high-resolution 3D imaging that reveals the distinct morphological features of various pathogens, such as the C. albicans cell wall and immune responses within the corneal stroma. Its ability to expand and clear tough tissues like the human cornea enables a new level of detail in pathogen identification, offering valuable insights for advancing diagnostic methods and therapeutic strategies for sight-threatening infections.
This Magnify image showcases the detailed ultrastructure of extracellular vesicles within human stem-cell-derived lung organoids. Captured with a Nikon Eclipse Ti2 equipped with a CSU-W1 spinning disk confocal module and Andor v.4.2 Zyla sCMOS camera, the sample was expanded 10.2-fold and stained with Alexa Fluor 488 NHS ester (green) and DiD (magenta). Panel b is 3D reconstruction of the EVs in panel a; Panel c shows a close-up 3D reconstruction of the highlighted EV in a; d is the orthogonal view of c.
By achieving resolutions down to 15 nm, Magnify allows for the precise visualization of complex internal structures within extracellular vesicles, which are crucial for understanding their roles in cellular communication, disease progression, and potential therapeutic targeting. Preserving lipids during expansion is key to maintaining the integrity of these vesicles, providing a clear view of their morphology and function. Such insights are essential for advancing our knowledge of how extracellular vesicles contribute to both normal physiology and pathological conditions.
These images compare tissue samples captured with and without Magnify processing using the same Cephla’s SQUID system setup, equipped with a CrestOptics X-Light V2 confocal spinning disk, IMX571 camera, and a 63×/1.2 water immersion objective. The samples were stained differently to highlight nuclear and vascular structures, with Magnify enhancing the resolution and clarity, making abnormalities more discernible.
Magnify’s enhanced imaging capability allows for the clear visualization of nuclear abnormalities, which are often linked to cancer, and provides critical insights into vascular pathologies such as tumor angiogenesis. By revealing these subtle yet significant details, Magnify aids in the early detection and study of cancerous changes and vascular developments, offering valuable tools for advancing cancer research and therapeutic strategies.
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