The Magnify Expansion mounted Slide is designed for immediate imaging, eliminating the need for any sample preparation processes.
It is ideal for microscopic demonstrations or as a first step in trying Magnify Expansion Microscopy; it helps users evaluate the capabilities of Magnify Expansion microscopy and become familiar with imaging expanded samples. This pre-made slide is also perfect for teaching Expansion Microscopy imaging techniques.
It is compatible with a wide range of imaging systems, including slide scanners, widefield fluorescence microscopes, and confocal microscopes in both upright and inverted configurations. The slide works well with dry lenses, water immersion lenses, and oil/silicone immersion lenses of any magnification, offering 3.5x to 11x expansion according to your needs. Each slide comes pre-stained with morphological or customized markers of your choice, ensuring that you can achieve optimal imaging results right out of the box.
This Magnify image was captured using a ZEISS Lattice Structured Illumination Microscopy (SIM) 5 with a 63× oil immersion objective (NA 1.4). The sample, an expanded human FFPE kidney tissue, was stained with ACTN4 (magenta) to highlight the tertiary podocyte foot processes. Magnify, combined with the SIM 5’s original 65 nm resolution, enhanced the imaging down to 6-10 nm, achieving clarity similar to Correlative Light and Electron Microscopy (CLEM) but with much simpler and quicker preparation.
This ultra-high-resolution capability is invaluable not only for renal pathology but also for a wide range of biomedical research areas, such as neurodegenerative diseases, cancer, and cardiovascular conditions. By providing detailed views of subcellular structures quickly and efficiently, Magnify empowers researchers with SIM scopes to explore complex biological processes, accelerating discoveries and advancing the development of more effective treatments.
This dataset was captured using Cephla’s SQUID, a system designed to eliminate barriers to high-end imaging, equipped with a CrestOptics X-Light V2 confocal spinning disk and IMX571 camera, paired with a 63×/1.2 water immersion objective. The mouse brain sample was stained with DAPI, synaptophysin (green), homer (red), and LEL (white), and imaged in high resolution to reveal the detailed structure of neuronal and synaptic components.
Magnify’s integration with accessible, high-throughput imaging systems like Cephla’s SQUID enables detailed visualization of synaptic structures without the need for bespoke setups. This approach enhances our ability to study synaptic organization and connectivity in the brain, providing critical insights into neuronal circuitry. By making advanced imaging more accessible, Magnify facilitates broader adoption of cutting-edge research techniques, driving discoveries in brain function and neurological disorders.
