The Magnify Expansion Kit (Optimized for Organoid Specimens) provides researchers with an easy-to-use system for achieving nanoscale imaging resolution on conventional microscopes using 3D organoid cultures. This kit contains reagents sufficient for five reactions, each suitable for processing multiple organoid samples [cite: user]. Utilizing an advanced hydrogel chemistry optimized for these complex 3D structures, the kit allows for isotropic physical expansion, typically up to 8-11 folds in deionized water. This expansion enables detailed visualization of intricate cellular arrangements and subcellular structures within the organoid specimen, resolving details previously beyond the diffraction limit. The Magnify process anchors and preserves diverse biomolecules within the organoids, facilitating comprehensive multi-scale analysis post-expansion. The included protocol offers specific guidance for processing fixed organoids, noting that homogenization times may vary depending on the organoid type and require user optimization. This product is developed and sold exclusively for research purposes.
