The Magnify Expansion Kit (Optimized for Freshly Preserved Tissues) provides researchers with an easy-to-use solution for achieving nanoscale imaging resolution on conventional microscopes using freshly preserved tissue slices. This kit contains reagents sufficient for five reactions, with each reaction designed to process multiple tissue samples, offering excellent value. Leveraging an advanced hydrogel chemistry, the kit facilitates isotropic physical expansion, typically up to 11-fold in deionized water, allowing detailed 3D visualization of subcellular structures and molecular components previously beyond the diffraction limit. The Magnify process anchors and preserves diverse biomolecules, including proteins and lipids, within the tissue during expansion. The kit includes optimized protocols and buffers tailored for freshly preserved specimens, such as Buffer H for tissue homogenization. Note that homogenization times may need optimization depending on the specific tissue type (e.g., brain vs. muscle tissue requires different incubation periods ). This product is developed, designed, and sold exclusively for research purposes.